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The new, enhanced, lattice of mineral bonds among soil particles forms between several hours and few days, depending on the desired final bond content. It comprises calcite crystals which grow in number and size following hierarchical plane expansion with their dimensions ranging from few, up to hundreds of micrometers The resulting material is lightweight cemented soil which retains the permeability of its matrix 4 , 13 exhibits significant enhancement of strength and stiffness 14 and is difficult to produce by any other synthetic means.

There is still uncertainty, however, about the design and application of a controlled, large-scale soil improvement mechanism based on MICP, despite the extensive knowledge established via laboratory experimentation 15 — Few attempts have been made at large scale targeting real geo-technical problems 22 — 24 and in some cases failed to reproduce the desired precipitation mechanism However, the role of the intrinsic properties of the base material on the formation of the bond lattice remains relatively underexplored.

To this purpose, in the present study we adopt two different base materials and subject them to identical external treatment conditions to ultimately evaluate whether and how complex bio-chemical and transport phenomena adapt to the different available porosities. Additionally, we focus on the study of kinetics of urea hydrolysis for the case of MICP induced via utilizing lyophilized cells.

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The use of this cell state, which is an alternative to the direct use of vegetative cells, is considered to facilitate significantly MICP applications by overcoming the need for complex on-site bacteria cultivation It further incorporates elements of economic efficiency by lowering energy requirements and needs in resources and equipment for in-situ applications. Moreover, it ensures that quality control tests are carried out prior to propagating solutions in the targeted soil volume.

Overall, MICP is rendered easier to reproduce, since the most crucial elements of the process, i. Finally, although the bio-cemented geo-material was brought into focus over a decade ago 4 , 9 its peculiar structure, result of bio-chemical and transport processes, has been treated solely in qualitative approaches, via surface and textural observations.

In the context of the current knowledge 27 — 29 microstructural information has been captured and outlined mainly through scanning electron microscopy SEM.

More precisely, the chemical composition of treatment solutions 27 , the environmental conditions 28 and the degree of saturation 29 during MICP improvement are all found to yield distinctive precipitation behaviors and correspond to varying obtained mechanical properties. Moreover, microstructural evidence provided in the literature is often limited to the scale of few grains and bonds and thus it remains unclear whether such evidence can be considered representative of the macro-scale.

To overcome such uncertainties regarding the limited validity of textural observations, micro-computed tomography micro-CT is considered herein as the ultimate tool which allows providing with a robust determination of micro-scale quantities. The goal is to capture spatial and morphological heterogeneities of micro-scale properties. Recent work in this direction has been reported in 30 where micro-CT is mobilized for capturing microstructural quantities and their evolution with respect to changes in rheological properties, such as the porosity, permeability and effective diffusion.

Contrary to 30 , the work presented herein deals with the micro-architecture of bio-cemented materials by implementing micro-CT to isolate and analyze individual bond particles which compose the calcite lattice in two different base materials. Results on the geometrical and spatial characteristics are interpreted and evaluated with respect to the captured strength and stiffness parameters. Moreover, complementary microstructural inspection tools are mobilized to further shed light on the bond growth mechanism and on the previously underexplored internal state of calcite bonds.

Kinetics of cell-free MICP Previous studies reported in the literature 5 — 24 have utilized extensively whole vegetative cells of Sporosarcina Pasteurii which are introduced in soils to ultimately induce their bio-cementation. Herein, we obtain ureolytic cells in dry powder form through a freeze-drying process and evaluate their ureolytic capacity upon rehydration, as described in the methods section.

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We thus contribute to the debate on the importance of factors affecting: i MICP efficiency, ii the mechanical response and iii peculiar micro-architecture of bio-improved geo-materials. D- , respectively, describe in detail the sampling, gas extraction, and