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However, studies have found that SALSA is also expressed sporadically in the endothelial cells of cardiac capillaries and in capillaries of skeletal muscle, at least in mice [ 23 , 24 ].
Several secreted forms of SALSA have been found in body fluids lining the mucosal surfaces such as saliva, lacrimal fluid, pancreatic juice and as well in bronchoalveolar lavage [ 15 , 16 , 25 , 26 ].
However, so far it has not been described in blood. Recent advances in the understanding of the physiological function of SALSA point towards a role in both epithelial cell differentiation and innate immunity [ 28 ].
The pathogenesis of pregnancy complications, in particular PE, has been associated with a regulatory disturbance in immune responses and specifically in complement activation.
Our approach aimed to elucidate the role of SALSA in pregnancy by measuring its levels in AF samples from women with normal and complicated pregnancies, localizing the SALSA protein in placenta and determining its related molecular targets and interaction partners in placental lesions.
Samples from second trimester All karyotypes and alpha-fetoprotein levels were normal. Samples from second trimester are referred to as early pregnancy.
Control AF samples from women with uncomplicated pregnancies gestational week Placental samples Placental samples were obtained from different cohorts.
The following paraffin-embedded tissue samples were obtained from Medical University of Graz, Austria. Frozen placental tissue from PE gestational weeks PE and healthy women delivered both by caesarean section and vaginal delivery.
Detailed description of the cohort has been reported previously [ 14 ]. All subjects gave written informed consents. SALSA purified from saliva was used as a protein concentration standard. For each sample a dilution series was made to ensure that the readings were within the linear range.
Measurements were based on a minimum of three separate assays.
DALSA SP PDF
Fluorescence microscopy and immunohistochemistry Sections of paraffin-embedded tissues were prepared as above. Classical and lectin pathway activation was investigated by staining with polyclonal rabbit anti-C4c Dako and Alexa labeled goat anti-rabbit Invitrogen diluted and in PBS, respectively. An overlay was performed with un-diluted AF after initial blocking on frozen sections. Following this the antibodies were added as described. After final incubation and wash, excess liquid was removed and a mounting liquid was added.
DALSA SP PDF
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This item may be a floor model or store return that has been used. High-performance, high-quality data-converter designs and IP blocks for the industrial, professional, scientific, imaging, and audio markets.Coagulation time was measured using a coagulometer.
During pregnancy there is a general suppression of the immune system to protect the fetus.
One of the main objectives of the Author in preparing this Paper was to help the Highway Engineers in the country to do the planning and design of culverts and small bridges for road projects correctly and expeditiously. Smart Cameras Compact, self-contained vision tools with embedded software.
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Frozen placental tissue from PE gestational weeks Sign dzlsa to check out Check out as guest. No exposure control.
Control AF samples from women with uncomplicated pregnancies gestational week Interest will be charged to your account from the download date if the balance is not paid in full within 6 months.
The protein is found to be associated with the epithelial layers in the lung, mouth, trachea, gastrointestinal tract, vagina and skin [ 18 — 22 ].