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The PCR products were analysed by electrophoretic separation and purified directly from the agarose gel 1. The purified KEX1 fragments were combined and mixed in a unique sample. The assays were incubated at room temperature for 1 h and the results were read in the Zenyth microplate reader Biochrom Anthos, Cambridge, UK. Based on the standard curve, the DNA concentrations of the P.
Then the quantification cycle Cq values of those dilutions were obtained by qPCR, in triplicates. A new standard curve P.
For the preparation of the P. The P.
Genotyping The P. Reference positive controls supplied by the manufacturer with four fluorochrome control peaks were used in the SBE assays: 29 nt cytosine D2—black ; 35 nt adenine D1—red ; 36 nt guanine D3—green ; and 50 nt thymine D4—blue. The maximum height values of fluorescence peaks observed in the SBE products were divided by the reference fluorescence values determined in each assay by the reference positive control.
This procedure allowed the calculation of the average normalized relative frequencies of each SNP allele in the studied pools. Statistical tests were applied to investigate associations at a significance level of 0. This procedure was specifically directed to the P. The KEX1 standard pattern serial dilutions were analysed by the PicoGreen method and concentrations were calculated using the standard curve for DNA concentration versus fluorescence, as described above.
This standard curve equation expresses the correlation between the concentration of P. After Koulibaly's death, his eldest grandson Mamari—also known as Biton—ruled the city and made it flourish. Today the town in some ways conserves the tradition and architecture of the ancient city.
Biton spread terror, organised the army, and cmdt pdf the association into a city. He expanded cmdt pdf territory from Segou Koro to Timbuktu.
It remained autonomous and paid tribute to Biton. After Biton's death inone of the Coulibaly family slaves, Ngolo Diarraobtained power to control the Bambara kingdom and established the Diarra dynasty.
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Diarra continued Biton's conquest and extended the kingdom from Guinea to Timbuktu. Ahmadu had to deal with Cmdt pdf rebellions and challenges from his brothers but he continued to rule until when insignia forum er town fell to French forces led by Colonel Louis Archinard.
The commune is subdivided into 15 quartiers: The city is irrigated by two important waterways: The rainy season starts in June and lasts about four months until September. The harmattan is the dominant wind in the dry season and it blows from north to south. The monsoon blowing from south to north-west is more frequent during rainy season hivernage.
With a rural population that is largely nomadic semi-sedentary or sedentary, the population consists of many ethnic groups, such as BambaraBozo, FulaniSoninkeMalinke and Toucouleur. Bambaras are mostly farmers and are the most numerous ethnic group.
Their language is Bambara or Djoula. The Bozos are the second most populous ethnic group.
They typically live near the shore of the Niger river, in small towns of cmdt pdf houses. The Bozo economy is based on fishing. Bozo people have a monopoly on the transport system because cmdt pdf their cmdt pdf of the Niger, its shallows and seasonal lakes, and are regarded as the masters of water. The Somonoalso fishermen, are not a distinct ethnic group but a mixture of Bambara, Bozo and Soninke. They share costumes, religious beliefs, and practices with the Bambaras.
The Soninke people are great travelers and Muslims, and have largely conserved their traditions.
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The Bambaras used to transmit their knowledge by oral tradition, hence much of their art and culture is unknown. The history of the Bambara state's traditional religious practices are ambiguous. They practice animism and fetishism as cultural practices, and also totemic cmdt pdf monism cult of ancestors. Women make the pottery by hand with the clay coming from the Niger River and bring the finished works to the local Monday market.
French Colonial and traditional Sudanese and neo-Sudanese. The Sudanese style influenced public building and important residences. Monuments and great cmdt pdf are also built according to this style.
Many of Sudano-Sahelian 's kings built imposing palaces in the cities cmdt pdf which they ruled and most of these buildings are in red clay. The materials used for building are generally quite poor and many of the buildings need to be restored to maintain their state. In the city center, the main landmark is the water tower.
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The most important economic activities are fishing, cattle herding and small scale farming. The main crop is rice pearl millet but sorghum Sorghum bicolor and black-eyed peas are also grown. The yields are generally low.
Commerce consists mostly of the small scale exchange and sale of products from the primary sector, sold weekly at the large Sudano-Sahelian market, drawing customers from far outside of the city.
The main products sold are vegetables, pottery, cotton, leather, fruit, ovens, cattle and cereals. The headquarters of the Office du Niger is based in the town.It also has useful online access. Views Read Edit View history.
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The cardiology chapter needs ECG and echocardiography pictures. They typically live near the shore of the Niger river, in small towns of cmdt pdf houses.
The frequencies of multiple P.
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